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Biochemist, Molecular Biologist, Research Expert Consultant Resume

Resume of DYH, Ph.D. Consultant

Principal Industries Served:

  • Biotechnology
  • Pharmaceutical

Education:

This expert has a Ph.D. in Biochemistry & Molecular Biology from the University of Medicine and Dentistry of New Jersey in Newark, NJ.

This expert received a B.A. in Liberal Studies/Chemistry from California State University in Fresno, CA.


Expertise and Services:

Biochemist, Molecular Biologist, Research Expert Consultant Resume
 

This expert specializes in protein purification using FPLC, HPLC, cloning, PCR, DNA purification, and DNA sequencing. Other expertise in protein purification includes gel shifts, western, northern, southern blotting, primer extension assays, cell culture, DNA and RNA transfection. Furthermore, this consultant has knowledge in membrane-based lateral flow diagnostic assays, membrane technology and applications, high throughput device design and optimization, life science applications, and environmental analysis.

Specialization of this consultant includes the molecular biology of human immunodeficiency virus replication, development/support of high throughput devices and applications for the genomics industry, molecular biology kits for DNA purification, and product validation. Other specialties include quality documentation and preparation of technical literature, technical support, sales, marketing, and technical services for genomics applications. Further expertise of this consultant includes quality control, product development, marketing communications, packaging, supplier quality, and purchasing.

This expert has extensive knowledge regarding generation, purification, and biophysical characterization of mutant proteins. Furthermore, this expert has a background in isolating HIV-1 pre-integration complexes, molecular modeling programs, protein structures, and human protein (BAF) involved in HIV integration. Other specific areas of specialty in this expert's background include mutational analysis of HIV-1 reverse transcriptase, amino acid residues that influence dNTP substrate binding and/or drug resistance, analytical instruments and equipment, water and soil analysis, and pesticides.

This expert has conducted numerous studies in areas including Glutamine 151 of Human Immunodeficiency Virus Type 1 reverse transcriptase and RNA directed DNA synthesis, The p51 subunit of Human Immunodeficiency Type 1 reverse transcriptase, polymerase activity loss due to mutation in the HIV-1 reverse transcriptase, and amino acid residues and HIV-1 reverse transcriptase. Other studies this expert has participated in include Glutamine 151 of HIV Type-1 reverse transcriptase and substrate selection, the reconstitution of HIV type 1 integration in vitro, hi speed plasmid isolation, insertion of six amino acids into the HIV-1 reverse transcriptase and heterodimer activity, and purification of BAC DNA templates.


Professional Experience:

Consultant, Kevin Kennedy Associates Inc.

Providing biochemistry consulting, molecular biology, research science, management, consulting, and related expertise to a wide variety of clients.

 

Molecular Biologist, Management Consultant

Was responsible for managing consultants and projects. Was responsible for marketing, advertising, and partnering activities. Was responsible for delivering customer training courses. Writing and editing services for clients.

 

Senior Scientist, Genomics and Molecular Biology, a Bioscience Company providing technologies for new therapeutic drug development

Served as Group leader for the development of molecular biology kits for DNA purification. Developed and launched a new product line of molecular biology kits, resulting in $2M of new sales in the first year. Managed product validation, quality documentation, and preparation of technical literature. Provided critical technical support for major accounts, which contributed to the closing of over $1M in new business. New customers included several of the largest public and private genome research centers. Served as in house consultant to sales, marketing, and technical services for entire range of genomics applications. Completed a technical seminar tour through Asia and Australia introducing novel platforms for DNA purification. Provided ongoing product training to Sales and Technical Support Groups in North America, Asia, Europe, and Australia. Negotiated with vendors for sourced reagents and collaboratively drafted quality plans for production and Q.C. testing. Worked closely with other groups/departments in coordinating product development such as marketing, marketing communications, packaging, supplier quality, purchasing, and sales.

 

Research Fellow, Dana-Farber Cancer Institute (Department of Cancer Immunology & AIDS) & Harvard Medical School (Department of Pathology), Boston, MA

Designed experimental strategies toward elucidation of HIV-1 integrationƒn (generation, purification, and biophysical characterization of mutant proteins). Developed novel means of isolating HIV-1 pre-integration complexes (PICs) through the insertion of epitope tags using overlapping PCR. Used molecular modeling programs to analyze protein structures, presented experimental data at local and international scientific meetings, and prepared manuscripts for publication in peer reviewed journals. Delivered oral presentation of research on a novel human protein (BAF) involved in HIV integration at the international meeting on retroviruses at Cold Spring Harbor National Laboratories. Supported by National Research Service Award (NRSA) Grant from the National Institutes of Health.

 

Graduate Student, University of Medicine and Dentristry of New Jersey, Graduate School of Biomedical Sciences, Newark, NJ

Thesis work consisted of mutational analysis of HIV-1 reverse transcriptase and specifically identification of amino acid residues that influence dNTP substrate binding and or/drug resistance (publications below).

 

Pesticide Chemist, Anametrix Laboratories, San Jose, CA

Maintained and operated analytical instruments and equipment. Acquired and analyzed data usingƒnspecialized software. Managed large projects for clients, implementing the highest level of quality control in environmental analysis. Designed a new system for tracking projects and streamlining production. Trained and managed employees.

 

Pesticide Chemist, The Twining Laboratories, Fresno, CA

Prepared water and soil samples for analysis by chromatographic methods. Maintained and operated analytical instruments in the pesticide division of an environmental chemistry lab. Trained and supervised employees.

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