Consultant, Kevin Kennedy Associates Inc.
Providing chemistry consulting, biochemistry consulting, biomedical research, and related expertise to a wide variety of clients.
Chemist, Biochemist, a major research university
Researched the Identification of Novel FMRP mRNA Targets in Neurodegenerative Disorders. Techniques Employed: mouse brain dissection, synaptosome preparation, neuronal cell culture, mouse breeding and flow cytometry. Researched the Post-Transcriptional Regulation of RhoB mRNA by the RNA Binding Protein HuR. Techniques Employed: cell culture and transfection, cell fractionation, northern and western blotting, immunoprecipitation, recombinant DNA cloning, RT-PCR, fluorescent microscopy and sequence alignment analysis. Researched the Isolation of Novel Early Response Genes from the Central Nervous System in a Mouse Epilepsy Model. Techniques Employed: bacterial protein expression, mRNA isolation, in vitro AUrich RNA selection by affinity chromatography, cDNA synthesis, cDNA library screening, BLAST homology searches. Additional Training Mentor to graduate and undergraduate students, training in mouse handling and preparation of R01 grant applications.
Postdoctoral Research Fellow, Department of Pathology & Laboratory Medicine and Institute on Aging, University of Wisconsin-Madison
Researched The Molecular Biology of Alzheimer’s Disease. Techniques Employed: particle mediated gene transfer, PBMC isolation, in vitro transcription and RNA purification, northern hybridization analysis, SDS-PAGE and ECL western blot analysis, ELISA, PCR, RNA gel mobility shift assays, cell culture, protein purification and tat-mediated protein transfection, protein kinase assays, sequence analysis
Postdoctoral Research Associate, Department of Chemistry and Biochemistry, University of Notre Dame
Researched Continuation of Dissertation Projects described below
Graduate Student, Biophysics, and Molecular Biology Training Program, Department of Chemistry and Biochemistry and Biochemistry, University of Notre Dame
Self-Directed Dissertation Project: The Phosphorylation of Xenopus Transcription Factor IIIA by an Oocyte Casein Kinase. Techniques Employed: in vitro phosphorylation, protein digestion and peptide isolation, amino acid hydrolysis, identification of phosphoamino acids by thin layer chromatography, oocyte isolation and in vivo radiolabeling of proteins, affinity purification of antibodies, immunoprecipitation, expression and purification of recombinant protein in E.coli, SDS-PAGE, autoradiography and densitometry Primary Dissertation Project: The Effect of Ribosomes on the Transcription of RNA Polymerase III Genes. Techniques Employed: protein and nucleic acid purification, isolation of eukaryotic ribosomes, electrophoresis, germinal vesicle isolation, preparation of whole cell and nuclear transcription extracts, DNase I and copper phenanthroline footprinting, quantitative footprinting, in vitro transcription assays in extract, gel mobility shift assays, western blot analysis, molecular cloning, hybridization analysis, DNA and RNA sequencing Additional Training Certification in radioisotope usage and small animal dissection.
